Analysis Note

ControlPOSTIVE CONTROL:

Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas.

Application

Immunohistochemistry (Frozen & fixed-paraffin embedded) 2-4 µg/mL 30 min at RT. Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.

ELISA (not suitable for coating, contains BSA) 1:200-1:2000

Not suitable for Western blotting


Optimal working dilution should be determined by the end user.

Anti-MMP-2 Antibody, pro & active form, clone A-Gel VC2 is an antibody against MMP-2 for use in ELISA, IH, IH(P).

Research CategoryCell Structure

Research Sub CategoryMMPs & TIMPs

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer or the stromal cells. There is a cooperation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction.

Immunogen

Human APMA-activated native 72kDa Gelatinase A.

Epitope: pro and active form

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1048

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide

Format: Purified

Specificity

MAB13431 recognizes proteins of 72kDa and ~66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72kDa collagenase IV or gelatinase A). CHEMICON MAB-13431 shows no cross-reaction with pro and active forms of other MMPs.

CELLULAR LOCALIZATION:

Cytoplasmic.

Storage and Stability

Antibody is stable for 12 months when stored at 2-8°C